Luis Suarez loses 888poker endorsement, but Barcelona still want to sign him
Online gambling firm 888poker said it had "decided to terminate its relationship" with "immediate effect".
Suarez joined 888poker as a worldwide ambassador shortly before the World Cup, and produced several video diaries for the website during the tournament, including one after his two goals in the 2-1 victory over England.
Suarez's boot deal with Adidas could also be in jeopardy after the sportswear manufacturer announced on Thursday it would consider its partnership with the player.
Meanwhile, Barcelona are still very interested in signing Suarez despite his four-month ban for biting Italy's Giorgio Chiellini during a World Cup match.
He is not allowed to train with his club or enter a football stadium.
Yet La Liga side Barca, who already have Argentina forward Lionel Messi and Brazil playmaker Neymar, would consider signing Suarez for the right price.